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. 2023 Feb 3;28(3):1482. doi: 10.3390/molecules28031482

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Viability and phenotype of ⁸⁹Zr-labelled human Tregs compared to mock-labelled Tregs. (A) Treg viability assessed following 72 h of culture after radiolabelling. Tregs were analyzed by flow cytometry for the percentage of cells expressing (B) CD4, (C) CD25, and (D) CD39 markers. Data are normalized to untreated Tregs. (Gray) ⁸⁹Zr-Tregs, (white) mock-labelled Tregs that do not contain ⁸⁹Zr; n = 3 for all experiments with error bars representing SD.