Figure 5.

Time-of-addition assay results for Saussurea costus acetic extract. Vero cells were infected at MOI 1.5 and incubated with the extract at different times during the infection, as described in the Materials and Methods. After 48 h post-infection, the virus genome was quantitated in the supernatant via qPCR. The percentage of inhibition was determined as the ratio between treated and untreated infected cells. Data are expressed as mean +/− SD for n = 3. Statistical analysis was performed using one-way ANOVA, followed by multiple comparisons testing with significance indicated as * p < 0.05, ** p < 0.01.