Table 2.

Effect of multi-nutrient supplementation on mating behavior and reproductive outcomes in Western diet-fed C57BL/6J mice.

Mating and Reproductive Outcomes	LF	HF	FE
Copulatory plugs (5-day total)	10	1 *	6
Copulatory plugs (% total females)	50%	6%	30%
Number of litters (total)	7	0 *	4
Copulatory success (%; Litters/Plugs)	70%	0%	67%

Harem-breeding of mice occurred in a 1:2 male-to-female ratio during a five-day period (LF: n = 20 females, HF: n = 18 females, and FE: n = 20 females). Vaginal plugs were counted at six a.m. each morning and females were singly housed following mating. LF = Low Fat Diet, HF = Western Diet (WD), and FE = ‘Fertility Enhancer’ multi-nutrient supplement with WD. * Significantly different from LF (p ≤ 0.05).