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. 2023 Jan 21;15(3):570. doi: 10.3390/nu15030570

Figure 3.

Figure 3

Cumulative food intake from one to four months of age of O-CON, O-WD and O-REV male and female animals treated with vehicle or leptin during suckling and fed SD or WD from weaning. Data are mean ± SEM (n = 8–12). Statistics: After data separation depending on post-weaning diet, three-way ANOVA was performed to analyze the effect of sex, maternal diet and/or leptin treatment. In each sex, two-way ANOVA was performed to analyze the effect of leptin treatment and/or maternal diet. Single comparisons between leptin versus vehicle-treated rats of all experimental groups were carried out using Mann-Whitney U test. Symbols: sex (S), maternal diet (MD), leptin treatment (L); A ≠ B (p < 0.05, LSD post-hoc, two-way ANOVA); *, different from their vehicle-treated equal (p < 0.05, Mann-Whitney U test). Abbreviations: offspring of CON-dams (O-CON), offspring of WD-dams (O-WD), offspring of REV-dams (O-REV), vehicle (V), leptin (L).