Figure 5.

ROS accumulation and MMP alteration induced by compound 14b. (A,B) The intracellular ROS detection by DCFH-DA after A549 cells treated with compound 14b at indicated concentrations for 12 h. (C,D) The detection of MMP monitored by JC-1 dye after A549 cells treated with compound 14b at the concentrations of 10, 20 and 50 nM for 12 h. The experiments were performed at least three times, and the results of the representative experiments are shown. Data are presented as mean ± SEM *** p < 0.001 vs. Control group; significant difference as compared with the control group by t test. Scar bar: 10 μm.