Figure 4.

Percentage (mean ± SEM, n = 3) cell viability (neutral red assay) in Hep-G2 (human hepatocellular carcinoma), PC-3 (human prostatic adenocarcinoma), and MC3T3-E1 (murine pre-osteoblast fibroblasts) treated for 24 h with native (circled lines) and micellar (squared lines) formulations of apple peel (Malus domestica cv. Anna), whole plum (Prunus domestica cv. Satsuma), or whole guava (Psidium guajava L). Lines with triangles show the effect of equivalent amounts of the empty micelle formulation on the cells. IC₅₀ (the concentration of the sample required to inhibit neutral red response to 50% respective to the untreated control) is shown as µg extract mL⁻¹. Statistical differences between native and micellar formulations within cell lines were determined by one-way ANOVA, followed by Bonferroni’s multiple comparison post-hoc tests, and they are indicated by superscript letters (p < 0.05) (GraphPad Prism v. 9.3.1., USA).