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. Author manuscript; available in PMC: 2023 Feb 11.
Published in final edited form as: Cell Rep. 2022 Dec 27;41(13):111875. doi: 10.1016/j.celrep.2022.111875

Figure 3. The increase in mtDNA content after L1 arrest does not require atfs-1-dependent nuclear transcription.

Figure 3.

(A) Images of wild-type, atfs-1(null), and atfs-1ΔNLS worms under control and L1 arrest-fed conditions, obtained when the control condition worms reached the L4 stage (scale bar, 0.5 mm).

(B) Quantification of body length of wild-type, atfs-1(null), and atfs-1ΔNLS worms under control conditions when the worms reached the L4 stage (n = 3 ± SD, **p = 0.0026, one-way ANOVA with post hoc Dunnett’s test).

(C) Quantification of body length of wild-type, atfs-1(null), and atfs-1ΔNLS worms under L1 arrest-fed conditions when the control worms reached the L4 stage (n = 3 ± SD, *p = 0.014 comparing wild-type and atfs-1DNLS worms and ****p < 0.0001 comparing wild-type and atfs-1(null) worms, one-way ANOVA with post hoc Dunnett’s test).

(D) mtDNA quantification of wild-type, atfs-1(null), and atfs-1ΔNLS worms when the control condition worms reached the L4 stage (n = 3–5 ± SD, ****p < 0.0001 comparing wild-type and atfs-1(null) worms and **p = 0.0097 comparing atfs-1(null) and atfs-1ΔNLS L1 arrest-fed worms, two-way ANOVA with post hoc Tukey’s multiple comparisons test).

(E) Time course of mtDNA quantification by qPCR comparing wild-type, atfs-1(null), and atfs-1ΔNLS worms fed for 48 h after L1 arrest (n = 4 ± SD for wild-type *p = 0.029 comparing wild-type and atfs-1(null) worm mtDNA at 48 h, *p = 0.035 comparing atfs-1(null) and atfs-1ΔNLS worm mtDNA at 48 h, two-way ANOVA with post hoc Tukey’s multiple comparisons test).

(F) Images of wild-type control and L1 arrest-fed worms treated with 0, 10, and 30 μg/mL of EtBr when the control worms reached the L4 stage (scale bar, 0.5 mm).

(G) Quantification of body length of wild-type control worms treated with 0, 10, and 30 μg/mL of EtBr, measured when worms treated with 0 mg/mL EtBr reached the L4 stage (n = 3, ****p < 0.0001, one-way ANOVA with post hoc Tukey’s test).

(H) Quantification of body length of wild-type L1 arrest-fed worms treated with 0, 10, and 30 μg/mL of EtBr when the worms treated with 0 μg/mL EtBr reached the L4 stage (n = 3, ****p < 0.0001, one-way ANOVA with post hoc Tukey’s test).