REAGENT or RESOURCE | SOURCE | IDENTIFIER |
---|---|---|
Chemicals, peptides, and recombinant proteins | ||
| ||
Ethidium Bromide | Invitrogen | 15585011 |
TMRE | Thermo Fisher | T669 |
| ||
Experimental models: Organisms/strains | ||
| ||
C. elegans: MQ130 (clk-1(qm30)) | Caenorhabditis Genetics Center (CGC) | RRID: WB Cat#: WBStrain00026639 |
C. elegans: CB1370 (daf-2(e1370)) | Caenorhabditis Genetics Center (CGC) | RRID: WB Cat#: WBStrain00004309 |
C. elegans: CB1038 (daf-16(mu86)) | Caenorhabditis Genetics Center (CGC) | RRID: WB Cat#: WBStrain00004840 |
atfs-1 ΔNLS | Shpilka et al., 2021,1 Yang et al., 202210 | N/A |
atfs-1 R/R | Shpilka et al., 20211 | N/A |
pdr-1(tm598) | National BioResource Project (Tokyo, Japan) |
N/A |
atfs-1(null) | Deng et al., 20195 | N/A |
PIE-1::GFP | Laboratory of Dr. Craig C. Mello | N/A |
GLH-1::GFP | Laboratory of Dr. Craig C. Mello | N/A |
vha-6p::tomm-20(1–54)::mScarlet | Laboratory of Dr. Gary Ruvkun | N/A |
| ||
Oligonucleotides | ||
| ||
Full list of qRT-PCR primers is presented in Table S2 |
This paper | N/A |
| ||
Software and algorithms | ||
| ||
Fiji ImageJ | ImageJ | RRID:SCR_002285 |
Prism | Graphpad | RRID:SCR_002798 |
OASIS2 | Han et al., 201645 | https://sbi.postech.ac.kr/oasis2/surv/ |
Biorender | Biorender | https://biorender.com/ |