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. Author manuscript; available in PMC: 2023 Sep 15.
Published in final edited form as: J Mol Biol. 2022 Oct 19;434(23):167859. doi: 10.1016/j.jmb.2022.167859

Table 2.

Summary of PTM effects on αS aggregation

Position Mutant Rate Aggregation Conditions Ref.
N-terminus Ac-αSa 0 PBS, 137 mM NaCl, pH 7.4, 140 μM αS, 600 rpm [168]
Lys6 K6Ub −1 10 mM PBS, 0.05% NaN3, pH 7.4, 100 μM αS, shaking [103]
Lys10 K10Ub −1 10 mM PBS, 0.05% NaN3, pH 7.4, 100 μM αS, shaking [103]
Lys12 K12Ub −1 10 mM PBS, 0.05% NaN3, pH 7.4, 100 μM αS, shaking [103]
Lys21 K21Ub −1 10 mM PBS, 0.05% NaN3, pH 7.4, 100 μM αS, shaking [103]
Lys23 K23Ub −1 10 mM PBS, 0.05% NaN3, pH 7.4, 100 μM αS, shaking [103]
Lys32 K32Ub −2 10 mM PBS, 0.05% NaN3, pH 7.4, 100 μM αS, shaking [103]
Lys34 K34Ub −2 10 mM PBS, 0.05% NaN3, pH 7.4, 100 μM αS, shaking [103]
Tyr39 nY39 −1 50 mM Tris, 150 mM NaCl, pH 7.5, 10 μM αS, 1000 rpm [99]
pY39 −2 20 mM Tris, 100 mM NaCl, pH 7.4, 100 μM αS, 1300 rpm [102]
Lys46 K46Ub −2 10 mM PBS, 0.05% NaN3, pH 7.4, 100 μM αS, shaking [103]
Thr72 gT72 −2 10 mM phosphate, 0.05% NaN3, pH 7.4, 50 μM αS, 1000 rpm [189]
Thr75 gT75 −2 10 mM phosphate, 0.05% NaN3, pH 7.4, 50 μM αS, 1000 rpm [189]
Thr81 gT81 −2 10 mM phosphate, 0.05% NaN3, pH 7.4, 50 μM αS, 1000 rpm [189]
Ser87 gS87 −2 10 mM phosphate, 0.05% NaN3, pH 7.4, 50 μM αS, 1000 rpm [187]
pS87, S129Ab −2 10 mM PBS, 0.05% NaN3, pH 7.4, 100 μM αS, shaking [164]
Lys96 K96 SUMO1 −2 10 mM sodium phosphate, 0.05% NaN3, pH 7.4, 50 μM αS, 1000 rpm [178]
K96 SUMO3 −1 10 mM sodium phosphate, 0.05% NaN3, pH 7.4, 50 μM αS, 1000 rpm [178]
K96Ub −2 10 mM PBS, 0.05% NaN3, pH 7.4, 100 μM αS, shaking [103]
Lys102 K102 SUMO1 −2 10 mM sodium phosphate, 0.05% NaN3, pH 7.4, 50 μM αS, 1000 rpm [178]
K102 SUMO3 −2 10 mM sodium phosphate, 0.05% NaN3, pH 7.4, 50 μM αS, 1000 rpm [178]
Tyr125 nY125 −2 50 mM Tris, 150 mM NaCl, pH 7.5, 10 μM αS, 1000 rpm [99]
pY125 −2 10 mM PBS, 0.05% NaN3, pH 7.4, 100 μM αS, shaking [162]
Ser129 pS129 −1 10 mM PBS, 0.05% NaN3, pH 7.4, 100 μM αS, shaking [162]
a

There is significant disagreement in the literature on the effect of N-terminal acetylation on aggregation.

b

While all other mutations and PTMs are presented as single site modifications, pS87 is included with the accompanying S129A mutation since those were the only data available. It is scored as −1 using the S129A aggregation rate as a pseudo-WT control to isolate the effect of Ser87 phosphorylation.