Table 2.
Localization of tetraspanins CD9, CD81, CD151, CD82, and CD63 in cow ovarian tissue, follicles, and oocytes
| Follicle stage | CD9 | CD81 | CD151 | CD82 | CD63 |
|---|---|---|---|---|---|
| Primordial | Oocyte area, granulosa cells | Oocyte area, granulosa cells | Oocyte area, granulosa cells | Oocyte area, granulosa cells | Oocyte area, granulosa cells |
| Primary | Oocyte area, granulosa cells | Oocyte area, granulosa cells | Oocyte area, granulosa cells | Oocyte area, granulosa cells | Oocyte area, granulosa cells |
| Secondary | Follicular epithelium, basement membrane, ovarian stroma | Follicular epithelium, basement membrane | Follicular epithelium, basement membrane | Follicular epithelium, basement membrane, oocyte area | Follicular epithelium, basement membrane, oocyte area |
| Tertiary | Follicular epithelium, theca layers, granulosa cells, antrum, cumulus cells, oocyte area | Follicular epithelium, theca layers, granulosa cells, antrum, cumulus cells | Follicular epithelium, theca layers, granulosa cells, antrum, oocyte area | Follicular epithelium, theca layers, granulosa cells, antrum | Follicular epithelium, theca layers, granulosa cells, antrum, cumulus cells, oocyte area |
| Oocyte | CD9 | CD81 | CD151 | CD82 | CD63 |
| Immature | mCD9: PM, filament-like structures passing through the ZP (Jankovicova et al. 2019), pCD9: the interrupted line on the PM with partial extension into the PVS (Jankovicova et al. 2019) | Clusters located along with the PM (Jankovicova et al. 2016) | Clusters lining up along the PM and/or in the PVS | Clusters in or near the PM filaments overlapping the ZP | Clusters in or near the PM filaments overlapping the ZP |
| Mature | mCD9: PM, filament-like structures passing through the ZP (Jankovicova et al. 2019), pCD9: the interrupted line on the PM with partial extension into the PVS (Jankovicova et al. 2019) | Clusters located along the PM (Jankovicova et al. 2016) | Clusters appertained to the PM | Clusters in or near the PM filaments overlapping the ZP, outer margin of the ZP | Clusters in or near the PM, filaments overlapping the ZP |
| Vitrified mature | mCD9: PM, filament-like structures passing through the ZP pCD9: the interrupted line on the PM with partial extension into the PVS | Clusters located along the PM | Clusters appertained to the PM | Clusters in or near the PM, filaments overlapping the ZP, outer margin of the ZP, cumulus cells | Clusters in or near the PM, filaments overlapping the ZP |
mCD9 monoclonal anti-CD9 antibody, pCD9 polyclonal anti-CD9 antibody, PM plasma membrane, ZP zona pellucida, PVS perivitelline space