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. 2023 Feb 11;14(2):107. doi: 10.1038/s41419-023-05652-z

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — A Cal27 cells were infected with a recombinant lentivirus containing CDK5RAP2 shRNA (CDK5RAP2 RNAi) or negative-control shRNA (Control), and then lysates of stable cells were immunoblotted with the indicated antibodies. B Colony-formation assay was performed using control and stable CDK5RAP2-knockdown cells. Colonies in each well were counted. C shRNA-carrying lines of Cal27 cells were injected subcutaneously into nude mice. The image shows isolated tumors. Mice were weighed every 4 days after injection, and the isolated tumors were weighed. Data are presented as means ± SD; ^***P < 0.001, two-tailed Student’s t-test.