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. 2023 Feb 11;14(2):106. doi: 10.1038/s41419-023-05657-8

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Western blotting showed expression level of total YAP and p-YAP in vector and DPT-OE cells. b total YAP and phosphorylated YAP (Ser127) were evaluated by western blot in ctrl and DPT-KO cells. c The mRNA levels of CTGF in DPT overexpression and vector cells. d The mRNA levels of CTGF in ctrl and DPT-KO cells. e Nuclear-cytoplasm separation assay indicated that DPT overexpression regulated YAP translocation between nuclear and cytoplasm. f Protein stability assay indicated the expression of YAP in different time points after Cycloheximide (CHX, 100 μg/ml) treatment in DPT overexpression and vector cells. g–i Immunoprecipitation showed the interaction between exogenous DPT and YAP proteins. j, k Immunoprecipitation assay showed the interaction between endogenous DPT and YAP proteins in BT549 and MDA-MB-231 cells. l Western blot showed that GFP-tagged YAP were precipitated by in vitro transcribed DPT in 293T cells. Right panel: graphic illustration of the YAP domain structure. ***P < 0.001, ****P < 0.0001.