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. 2023 Feb 11;14:779. doi: 10.1038/s41467-023-36550-9

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Illustration of the experimental procedure in vitro. b The mechanism illustration of DC maturation. c, ELISA assay for TNF-α, IL-12p40 and IFN-γ proinflammatory cytokines (n = 4 biological replicates per group). d Profile of DC maturation by FCM (n = 3 biological replicates per group). e–g Real-time in vivo and ex vivo IVIS imaging of Nano-CD after intravenously post i.v. injection of Nano-CD at 8 h, 12 h, 24 h, 48 h and 72 h. The fluorescence intensity of tumor tissues was also quantified. Data are presented as the mean ± s.d. (n = 5 biological replicates per group). In panels c, d, and g, data are presented as the mean ± s.d. and statistically analyzed using one-way ANOVA and Tukey’s tests.