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. 2023 Feb 11;13:2452. doi: 10.1038/s41598-023-29287-4

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The immunoreactivity of TH in the SN and ST of various experimental groups was examined using immunohistochemistry under the florescence microscope (20×). Rotenone intoxicated group showed less immunoreactivity than control (p < 0.001) in SN and ST. While, increased immunoreactivity of TH was observed in case of OLE treated group (16 and 32 mg/kg bwt) in SN (p < 0.001) and ST (p < 0.01). In all these three biochemical parameters, the OLE doses of 8 mg/kg bwt. showed a less significant effect. (*p < 0.05). Values are expressed as mean ± SEM (n = 6). ***p < 0.001, and *p < 0.05. SEM Standard error of mean, OLE Oleuropein, SN Substantia nigra, TH Tyrosine hydroxylase.