Figure 5.

Expression of α-Tubulin analyzed by (A) qRT-PCR using GAPDH as internal control and expressed in terms of relative mRNA expression, (B, C) Western blotting using β-actin as internal control and expressed in terms of relative densitometric value, (D) immune fluorescent staining and confocal microscopy to observe cellular localization. The representative blots of replicates were presented here. The full-length original images (cropped) of representative blots were presented in the supplementary Fig. S6 of Supplementary information file. Data was represented as mean ± SEM., n = 3, p < 0.05. #, δ and θ represent; compared to control group, TSA treated group and DNMT1 siRNA transfected group respectively.