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. 2023 Feb 11;13:2458. doi: 10.1038/s41598-023-29712-8

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of E-cadherin, N-cadherin and vimention analyzed by (A) qRT-PCR using GAPDH as internal control and expressed in terms of relative mRNA expression. Western blot analysis of E-cadherin, N-cadherin and vimention using β-actin as loading control, showing (B) effect of knockdown of DNMT1 and inhibition of HDAC1 & HDAC2 synergistically or due to either of any one on EMT, (C) relative densitometric value obtained from densitometric scanning after normalization with β-actin. The representative blots of replicates were presented here. The full-length original images (cropped) of representative blots were presented in the supplementary Fig. S8 of Supplementary information file. Data was represented as mean ± SEM., n = 3, p < 0.05. #, δ and θ represent; compared to control group, TSA treated group and DNMT1 siRNA transfected group respectively.