Figure 2.

JA insensitive mutants fail to acclimate to HL stress or induce a ROS wave response following a local treatment of HL or wounding. A, Arabidopsis plants were subjected to a HL stress or wounding treatment applied to a single leaf (L, Local), and ROS accumulation was imaged, using H₂DCFDA, in whole plants. Representative time-lapse images of whole plant ROS accumulation in WT and coi1 plants are shown alongside box plots of combined data from all plants used for the analysis at the 0- and 30-min time points (L and systemic [S] leaves). B, Similar to (A), except for the aos1-1 mutant. C, Ion leakage measurements of L and S leaves in Col-0 and coi1 plants following HL stress. Local and systemic leaves that were exposed to an extended period of HL stress with no pretreatment (highlight), pretreated with HL for a short period of time and allowed to incubate prior to extended light exposure (local acclimated, La; and systemic acclimated, Sa), and control plants receiving no pretreatment, were measured. D, Similar to (C), except for the aos1-1 mutant. All experiments were repeated at least three times with three plants per repeat. Two-way ANOVA followed by Tukey’s post-hoc test was conducted for statistical analysis. Letters represent a statistically significant difference of at least P < 0.05. Results for each experiment are displayed as box-and-whisker plots, with the borders corresponding to the 25th and 75th percentiles of the data. Each data value is included as a point within each box plot, with the horizontal line representing the median and “X” corresponding to the mean. Whiskers represent 1.5 times the minimum and maximum of the mean (1.5 times of the interquartile range).