Figure 4.
JA suppresses the ROS wave while SA augments it in response to either HL or wounding of a local tissue. A, Arabidopsis plants were untreated or pretreated with JA, subjected to wounding (Top) or a HL stress (Bottom) applied to a single leaf (L, Local), and ROS accumulation was imaged, using H2DCFDA, in whole plants. Representative time-lapse images of whole plant ROS accumulation in treated and untreated WT plants are shown alongside box plots of combined data from all plants used for the analysis at the 0- and 30-min time points (L and systemic [S] leaves). B, Similar to (A), except that plants were untreated or pretreated with SA before wounding treatments or HL stress were applied. C, Similar to (A) (Top), except for the aos1-1 mutant. D, Similar to (A) (Bottom), except for the aos1-1 mutant. All experiments were repeated at least three times with three plants per repeat. Two-way ANOVA followed by Tukey’s post-hoc test was conducted for statistical analysis. Letters represent a statistically significant difference of at least P < 0.05. Results for each experiment are displayed as box-and-whisker plots, with the borders corresponding to the 25th and 75th percentiles of the data. Each data value is included as a point within each box plot, with the horizontal line representing the median and “X” corresponding to the mean. Whiskers represent 1.5 times the minimum and maximum of the mean (1.5 times of the interquartile range).