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. 2023 Jan 30;69:101683. doi: 10.1016/j.molmet.2023.101683

Figure 6.

Figure 6

Muscular activity (shivering) shows no major difference between strains. EMG and MMG measurement of muscular activity were performed on AJ and B6 mice adapted to thermoneutral temperature (30 °C; WA) and consecutively exposed to cold (6 °C) for 2 and 7 days (CA) (n = 5–8). (A) Comparison of EMG and MMG signals from mice exposed acutely to cold showing both raw data and root mean square of these data. (B) Representative MMG measurement showing 20 s of low-intensity signal (i.e. without occurrence of physical activity) in each of the experimental conditions. (C) Quantification of low-intensity muscular activity (i.e. 15s-long signal out of 4 min MMG record with minimal or no physical activity). (D) Mean total muscular activity (i.e. including also muscle work). (C–D) ∗∗p < 0.01, ∗∗∗p < 0.001 vs. the respective WA group, ###p < 0.001 vs. the respective group after 2 days in cold (repeated measures mixed effect model (REML) and Tukey's multiple comparison test); @@@ p < 0.001 between the indicated groups (Sidak's multiple comparison test). Values for each data point can be found in S1 Data.