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. 2023 Jan 30;14:1094877. doi: 10.3389/fmicb.2023.1094877

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Copyright © 2023 Nisar, Ross, Brown, Bentham, Best and Whiley.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Viability based flow cytometry-cell sorting and qPCR assay. L. pneumophila grown on BCYE-GVPC agar at 37°C were used as a positive control and cells treated at 75°C for 10 min were used as a negative control. (A) Cytograms represent three populations: thiazole orange-stained alive (blue), thiazole orange-stained injured (green) and propidium iodide-stained dead (orange) cells. Propidium iodide selectively stained dead cells. Pink colored events are debris. (B) qPCR assay of thiazole orange stained alive and injured cells. The results showed that thiazole orange (TO) does not interfere with DNA extraction and qPCR.