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. 2023 Jan 30;14:1094877. doi: 10.3389/fmicb.2023.1094877

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Copyright © 2023 Nisar, Ross, Brown, Bentham, Best and Whiley.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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FISH of A. polyphaga stained with Alexa Fluor 647 labeled EUK1209 (red) and Legionella stained with Alexa Fluor 488 labeled LEG705 (green) probes. These micrographs show intracellular proliferation of environmental (shower water sample 1: A and sample 2: B) non-culturable Legionella within A. polyphaga.