Table 2.
UtrN-R3 versus DysR8-15 conformational stabilities measured via CD and AFM
| Construct | Origin | Tm1 (°C) | Tm2 (°C) | p-value | Unfolding forces (pN) | p-value |
|---|---|---|---|---|---|---|
| UtrN-R3 | Insect | 50.0 ± 0.5 | − | − | 122.3 ± 2.8 | − |
| UtrN-R3 | Bacteria | 48.8 ± 1.1 | − | 0.6122 | 87.3 ± 2.9 | 0.0004 |
| DysR8-15 | Insect | 54.8 ± 0.1 | 80.4 ± 2.6 | - | 95.5 ± 2.1 | 0.0134 |
| DysR8-15 | Bacteria | 55.2 ± 0.4 | - | 0.9653 | 100.1 ± 7.0 | 0.0188 |
Values for Tm are mean ± SEM.
Values for unfolding forces are median averages ± SEM.
N = 3 for Tm and N ≥ 4 unfolding forces for each construct. Statistics were performed using one-way ANOVA, with p < 0.05 considered significant. All statistics were performed on biological replicates (N).
Reported Tm p-values are bacterial construct compared against their insect counter part.
Reported unfolding forces p-values are the construct compared against UtrN-R3 WT insect.