. 2022 Dec 9;102(3):102409. doi: 10.1016/j.psj.2022.102409

Table 8.

Summary on raw data processing (n = 6/group).

Items	Control group	Group Ⅰ	Group Ⅱ	P value
Raw Reads	71,796.33 ± 6,946.93	73,034.00 ± 7,595.15	68,068.33 ± 4,602.79	0.65
Clean Reads	66,592.33 ± 6,416.54	67,540.67 ± 7,092.84	62,797 ± 4,285.66	0.62
Effective Reads	62,356.33 ± 6,123.90	63,175.00 ± 6,723.42	58,859.33 ± 3,881.90	0.64
AvgLen(bp)	252.00	252.00	252.00
GC, %	53.22 ± 0.15^b	53.43 ± 0.25^ab	53.71 ± 0.31^a	0.12
Q20, %	99.82 ± 0.00^b	99.84 ± 0.02^ab	99.85 ± 0.01^a	0.49
Q30, %	99.09 ± 0.01^b	99.14 ± 0.04^a	99.85 ± 0.01^a	0.03
Effective, %	86.84 ± 0.20	86.49 ± 0.45	86.48 ± 0.15	0.30

Note: Data estimation on each sample were shown in the following table, where Sample ID: Sample ID; Raw Reads: Counts of raw reads; Clean Reads: Counts of clean reads (post quality control and assembly); Effective Reads: Counts of effective reads after chimeric reads removal; AvgLen (bp): Average read length of each sample; GC(%): GC content, i.e., proportion of G and C in all bases; Q20(%): Percentage of bases with Q-score larger or equal to Q20; Q30(%): Percentage of bases with Q-score larger or equal to Q30; Effective(%): Percentage of effective reads in raw reads. In the same row, no letter or the same letter superscripts: no significant difference (P > 0.05). Different small letter superscripts: significant difference (P < 0.05).