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. 2023 Jan 18;19:100555. doi: 10.1016/j.mtbio.2023.100555

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© 2023 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 2 — Intracellular uptake and cytotoxicity. (a–c) Intracellular uptake of R@LIP/PFOB and IR-R@LIP/PFOB nanoliposomes (labeled with DiI) detected by CLSM (scale bars = 25 μm) and flow cytometry after various intervals of coincubation (n = 3, ∗∗∗P < 0.001). (d) Mitochondrial location of R@LIP/PFOB and IR-R@LIP/PFOB nanoliposomes (labeled with DiI) as monitored by Mito-Tracker (scale bar = 50 μm). (e) CCK-8 results after various treatments. (n = 5, ∗∗∗P < 0.001). (f) CLSM images of 4T1 cells stained by Calcein AM/PI after different treatments (scale bar = 100 μm).