Fig. 3.

In vitro ICD and DCs maturation. (a) CLSM images of CRT and HMGB1 exposure of 4T1 cells after various treatments (scale bars ​= ​50 ​μm). (b) ATP in 4T1 suspensions after various treatments as detected by the ATP kit. (n ​= ​3, ∗∗∗P ​< ​0.001). (c–d) The proportion of mature DCs (CD11c⁺CD80⁺CD86⁺) as detected by flow cytometry after different treatments (n ​= ​3, ∗∗P ​< ​0.01, ∗∗∗P ​< ​0.001). (e–f) Cytokine levels of TNF-α and IL-6 in DC suspensions were detected by the corresponding ELISA kits (n ​= ​3, ∗∗P ​< ​0.01, ∗∗∗P ​< ​0.001).