Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Essential 6 differentiation medium (day 2–3)
| Reagent | Final concentration | Volume (for 1 well of a 6-well plate) |
|---|---|---|
| Essential 6 medium | N/A | 2 mL |
| VEGF 80 μg/mL | 80 ng/mL | 2 μL |
| Basic FGF 100 μg/mL | 25 ng/mL | 0.5 μL |
| SCF 100 μg/mL | 50 ng/mL | 1 μL |
| SB431542 20 mM | 2 μM | 0.2 μL |
| Total | N/A | 2 mL |
Essential 6 medium can be stored at 2°C–8°C for up to 6 months. Upon cytokine reconstitution, Essential 6 differentiation medium has to be used immediately.
