StemPro-34 differentiation medium (day 4–5)

Reagent	Final concentration	Volume (for 1 well of a 6-well plate)
StemPro-34 complete medium	N/A	2 mL
VEGF 80 μg/mL	80 ng/mL	2 μL
Basic FGF 100 μg/mL	25 ng/mL	0.5 μL
SCF 100 μg/mL	50 ng/mL	1 μL
SB431542 20 mM	2 μM	0.2 μL
Total	N/A	2 mL

StemPro-34 complete medium can be stored at 2°C–8°C for up to one month. Aliquots can be stored at −20°C for up to 6 months. The aliquoted StemPro-34 complete medium can be stored at 2°C–8°C for up to one month after thawing. Do not re-freeze. Upon cytokine reconstitution, StemPro-34 differentiation medium has to be used immediately.