StemPro-34 differentiation medium (day 8–17)

Reagent	Final concentration	Volume (for 1 well of a 6-well plate)
StemPro-34 complete medium	N/A	1 mL
SCF 100 μg/mL	50 ng/mL	0.5 μL
IL-3 100 μg/mL	50 ng/mL	0.5 μL
Flt-3 100 μg/mL	50 ng/mL	0.5 μL
TPO 50 μg/mL	5 ng/mL	0.1 μL
Total	N/A	1 mL

StemPro-34 differentiation medium (day 8–17) is freshly prepared before usage.