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. 2023 Jan 20;29:100454. doi: 10.1016/j.pacs.2023.100454

Fig. 4.

Fig. 4

Mouse MSOT imaging and data analysis. (a) Cryoimage of a CD-fed mouse. (b) MSOT image recorded at 930 nm of the same mouse. (c) Cryoimage of an HFD-mouse. (d) 930 nm-MSOT image of the same mouse as in (c). The dashed white line marks the skin surface; the dashed yellow lines delineate the subcutaneous fat (SAT) region and the dashed orange lines the liver region. (e-f) Normalized ROI/BGR ratio spectrum within the SAT region for a CD-fed (e) and an HFD-mouse (f). (g) Mean (± standard error of the mean) normalized ROI/BGR ratio spectra for the whole cohort of the CD-fed (gray) and the HFD-fed (orange) mice. (h) Normalized ROI/BGR mean pixel intensity ratio at 930 nm within the SAT region for CD-fed (gray) and HFD-fed (orange) mice. (i-j) Normalized ROI/BGR ratio spectrum within the liver region of a CD-fed (i) and an HFD-mouse (j). (k) Mean (± standard error of the mean) normalized ROI/BGR ratio spectra for the whole cohort of the CD-fed (gray) and the HFD-fed (orange) mice. (l) Normalized ROI/BGR mean pixel intensity ratio at 930 nm within the liver region of CD-fed (gray) and HFD-fed (orange) mice. ns = non statistically significant, **p < 0.01.