Figure 3.

Improved immune activation in the tumor sites following immunotherapy with SRA-silenced DCs carrying the chaperone vaccine. (A) Mice with established B16-gp100 tumors were immunized with SRA-silenced DCs loaded with the hsp100-gp100 complex vaccine, followed by PCR analyses of immune-related genes in tumor tissues. (B) The levels of IFN-γ was also examined using ELISA. (C) Tumor infiltrating by CD4⁺ or CD8⁺ T cells as well as NK cells was analyzed using flow cytometry. (D) Tumor tissues from treated mice were subjected to TUNEL assays for tumor cell death. Bar = 50 um. The results are representative of three independent experiments. *p < 0.05; **p < 0.01.