Supplementary Figure 3.

DR^SERT neurons or 5HT levels in its projections are insensitive to ghrelin. (A–C) Fiber photometry-based measurement of in vivo DRN^SERT neuronal activity in response to ghrelin. (D–H) Fiber photometry signal of DRN^SERT axonal terminals in LH (E,F) and in BNST (G,H) in response to i.p. injection of ghrelin hormone (I–M) In vivo fiber photometry measurement of 5HT_2h sensor signal in LH (J,K) and BNST (L,M) in response to i.p. ghrelin hormone injection (n = 4–5 mice/group, down arrow indicates ghrelin injection time).