Supplementary Figure 5.

Activation of DRN^SERT → LH projection causes anxiety-related behavior and aversion in mice. (A–C) Schematic illustration of real time place preference (rtPP) test to measure aversion. Laser is delivered on the ‘on’ side of the chamber (A). Average heat map image summarizing duration spent for ChR2 and control mice in rtPP for 30 min (B). Summary bar graph quantification of time in active zone. (D,E) Elevated plus maze (EPM) test to measure anxiety level. Representative heat map images from ChR2 and control mice in the EPM setup for 5 min (D). Bar graph of time in closed arm (E). (F,G) Open field test (OP). Average heat map image of activity of ChR2 and control mice in the OP setup for 10 min (F). Bar graph of time in center zone (G) (N = 7–8 mice/group, ∗p < 0.05, ∗∗∗p < 0.001, Student's t-test).