Supplementary Figure 6.
5HT1B agonist inhibits release from AgRP synaptic terminals in PVN. (A) Schematic ChR2 assisted evaluation of ARCAgRP → PVN synaptic connection in Agrp-ires-cre mice. (B) Representative peak amplitude traces of whole-cell voltage clamp recordings from PVN neurons showing synaptic currents in response to AgRP axonal stimulation (blue lines) before and after CP-94253 perfusion. (C) Bar graph of normalized peak amplitude in baseline and after CP94253 administration (n = 10 neurons, ∗p < 0.05, Student's t-test).