Figure 1.

Stimulation of DRN^SERT axons suppress feeding through multiple forebrain targets. (A) Schematic diagram for stimulation of serotonergic axonal projections from DRN to LH, BNST, and PVN (upper). ChR2 expression in DRN^SERT neurons in Sert-cre mice (lower). (B–D) Representative photomicrograph showing ChR2 expressing DRN^SERT axons and dark onset food intake in response to DR^SERT:ChR2 axonal photostimulation in LH (B), BNST (C), and PVN (D) of ad libitum-fed mice areas. N = 8–18 mice, Two-way repeated measures ANOVA; ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001; (FT, fiber track, yellow dashed line, scale: 200 μm). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)