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. 2022 Dec 17;24(3):191–207. doi: 10.1111/mpp.13286

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© 2022 The Authors. Molecular Plant Pathology published by British Society for Plant Pathology and John Wiley & Sons Ltd.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Double‐stranded RNA (dsRNA) either associates externally or is taken up by Austropuccinia psidii and Coleosporium plumeriae urediniospores but not teliospores. Spores were incubated with 1 μg of Cy3‐labelled green fluorescent protein dsRNA on 1% water agar for 6 h (d) or 8 h (a–c,e,f) and were then treated with 75 U of micrococcal nuclease and Cy3 fluorescence observed using a Zeiss LSM700 confocal microscope. A. psidii urediniospores and teliospores are shown before (a) and after (b, c) micrococcal nuclease treatment. (a) A mixture of A. psidii urediniospores and teliospores, (b) A. psidii urediniospores and (c) A. psidii teliospores. C. plumeriae urediniospores at different stages of germination are shown after micrococcal nuclease treatment (d–f). Scale bars represent 20 μm (a–d), 5 μm (e), and 20 μm (f), respectively.