Figure 5.

Time course of LN229 and LN18 GFP-tagged GBM spheroids invading into brain tissue ex vivo. (A) A schematic displaying the approximate location of GFP-tagged spheroids sequentially implanted onto brain slices and cultured ex vivo for the indicated amount of time. (B) Representative images of LN229-GFP and LN18-GFP tagged GBM spheroids invading into brain slices ex vivo for 0, 1, 3, 5, and 8 days in vitro (DIV). Brain slices were co-stained for GFAP (astrocytes). Scale bar = 200 µm. (C–E) Quantification of the (C) % area of spheroid within brain slice, (D) the invasive area (µm²), and (E) the average particle distance from the nearest edge of the ellipse (n= 4 - 8 spheroids per condition implanted on slices collected from ≥4 mice; 3 independent experiments).