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. 2023 Jan 26;13:976945. doi: 10.3389/fonc.2023.976945

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Copyright © 2023 Decotret, Shi, Thomas, Hsu, Pallen and Bennewith

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Different invasive phenotypes of GBM cells when embedded into Matrigel versus murine brain tissue. (A, B) Representative images of LN229-GFP and LN18-GFP spheroids either embedded within 5.0 mg/mL Matrigel in vitro (in Matrigel), seeded on top of a thin layer of Matrigel in vitro (on Matrigel), seeded on top of ex vivo brain slice and imaged in the X/Y direction (Brain – top), or seeded on top of an ex vivo brain slice and imaged in the Z direction (Brain – side) on Day 5. Images of spheroids embedded in or seeded on top of Matrigel were imaged using a Zeiss AxioVert S100 fluorescence microscope with a 10X/0.25 objective. Spheroids seeded on top of brain slices were imaged using the Zeiss LSM 800 confocal microscope with a 20X/0.8 objective. The boxed regions are enlarged below each image. Scale bars = 200 µm.