Figure 3.

Coexpression of S12 with α4β2nAChR did not affect surface α4β2 expression. (a) Immunofluorescent staining of nonpermeabilized HEK293 cells stably expressing α4β2nAChR that were transiently transfected with cDNA encoding YFP (control) or also with S12 (+S12). Surface β2nAChR was detected using anti-β2nAChR antibody (1:200, Invitrogen PA5-77508). Boxed areas highlight representative transfected cells (yellow) for which the β2nAChR labeling intensity (red) was measured. (b) Quantified relative mean intensities of anti-β2nAChR staining show no significant difference in surface β2nAChR labeling between the +S12 and control groups. Data collection was performed ∼36 h after transfections. Data for each group were from two independent experiments with a total number of cells n = 1537 (control) and n = 1289 (+S12). Data are the mean ± SEM; p > 0.05 (ns) from two-tailed unpaired t test.