TABLE 1.

Mitochondrial PCR Primers and Probes

Primer/Probe	Nucleotide Sequence (5′ to 3′)	Tm, °C	Location Within ND5
Human 5′ primer	CAGCAGCCATTCAAGCAATGC	57.9	497–517
Human 3′ primer	GGTGGAGACCTAATTGGGCTGATTAG	58.8	666–691
Human probe	TATCGGCGATATCGGTTTCATCCTCG	59.9	528–553
Bovine 5′ primer	CAGCAGCCCTACAAGCAATGT	58.1	497–517
Bovine 3′ primer	GAGGCCAAATTGGGCGGATTAT	58.0	666–687
Bovine probe	CATCGGCGACATTGGTTTCATTTTAG	56.9	528–552

ND5, NADH dehydrogenase subunit 5; Tm, melting temperature.