TABLE 1.
Characteristics of study participants and QC samples included in the proteomics profiling experiments: blinded replicates, delayed processing, and within-person reproducibility over time (1 and 10-years)
| Blinded replicates | Delayed processing | Within-person reproducibility over 1-year | Within-person reproducibility over 10-years | |
|---|---|---|---|---|
| Total number samples (QC and participant), n | 28 | 46 | 84 | 80 |
| Standard QC samples, n | 2 | 2 | 2 | 2 |
| Number of replicates | 2 | 2 | 2 | 2 |
| Study participants, n | 12 | 14 | 40 | 38 |
| Plasma samples per participant | 2 | 3 | 2 | 2 |
| Plasma sample anticoagulant | ||||
| EDTA | 6 | 7 | 0 | 0 |
| Heparin | 6 | 7 | 40 | 38 |
| Participant characteristics | ||||
| Age (years), mean (range) | 46 (30–69) | 41 (24–57) | 45 (37–51) | 59 (48–67) |
| Female (%) | 50 | 79 | 100 | 100 |
| White (%) | 83 | 79 | 95 | 100 |
| BMI (kg/m2), mean (range) | 24.6 (19.5–32.0) | NA | 27.0 (18.3–50.0) | 24.0 (17.4–35.9) |
| Smoking (%) current | 0 | 7 | 0 | 3 |
| Time since last meal | ||||
| <8 h (%) | 50 | 64 | 29 | 21 |
| ≥8 h (%) | 50 | 36 | 71 | 79 |
BMI, body mass index; EDTA, ethylenediaminetetraacetic acid; QC, quality control.