KEY RESOURCES TABLE.
| Reagent or resource | Source | Identifier |
|---|---|---|
| Antibodies | ||
| Hamster anti-DLL1 | Genetex | HMD1-5 RRID:AB_11176107 |
| Mouse anti-alpha-Tubulin | Sigma-Aldrich | T5168 RRID:AB_477579 |
| Rat anti-Dll1 | Millipore Sigma | PGPM-1F9 MABN2284 |
| Rat anti-Notch1 | Cell Signaling Technologies | 5B5 mAb #3447 RRID:AB_2153498 |
| Rabbit anti-NICD | Cell Signaling Technologies | D3B8 # 4147 RRID:AB_2153348 |
| Alexa Fluor 680 Goat Anti-Mouse IgG | Thermo Fisher |
A28183 RRID:AB_2536167 |
| IRDye 680RD Goat Anti-Rat IgG | LI-COR | RRID:AB_10956590 |
| IRDye 800CW Donkey Anti-Mouse IgG | LI-COR | RRID:AB_621847 |
| IRDye 800CW Donkey Anti-Rabbit IgG | LI-COR | RRID:AB_621848 |
| Mouse Anti-Myc | Sigma | M4439 RRID:AB_439694 |
| AP-conjugated anti-rabbit secondary antibody | Cell Signaling Technologies | 7054 RRID:AB_2099235 |
| rabbit anti-HA | Cell Signaling Technologies | C29F4 RRID:AB_10693385 |
| rabbit anti-GFP N-term | Sigma |
G1544 RRID:AB_439690 |
| Goat anti-Hamster Alexa Fluor 488 | Thermo Fisher | A-21110 RRID:AB_2535759 |
| Bacterial and Virus Strains | ||
| Biological Samples | ||
| Chemicals, Peptides, and Recombinant Proteins | ||
| Streptavidin-IRDye800 conjugate | Rockland | S000-32 |
| Critical Commercial Assays | ||
| Click-iT Protein Reaction Buffers Kit | Thermo Fisher | C10276 |
| Dual Luciferase assay | Promega | E1960 |
| Click-IT™ Fucose Alkyne | Thermo Fisher | C10264 |
| Deposited Data | ||
| Experimental Models: Cell Lines | ||
| HEK293-T cells | ATCC | RRID:CVCL_0063 |
| Flp-in TRex 293 Cell Line | ThermoFisher | R78007 |
| NIH3T3 cells | ATCC | RRID:CVCL_0594 |
| Experimental Models: Organisms/Strains | ||
| Dll3pu Mus musculus | Jackson Laboratories | MGI:2173255 |
| Lfngtm1Rjo Mus musculus | Randy Johnson | MGI:3777244 |
| Oligonucleotides | ||
| Recombinant DNA | ||
| pSecTag2 Hygro C | ThermoFisher | |
| pcDNA6/TR | ThermoFisher | |
| pEF-BOS-rDll3-HA | Ladi et al. 2005 | |
| pcDNA5-TO-Kozak-hDll1-Cerulean | LeBon et al. 2014 | |
| pcDNA3.1Zeo(+) | ThermoFisher | |
| pcDNA3-Lfng-HA | Shifley and Cole 2008 | |
| pGA981-6 | Minoguchi et al., 1997 | |
| Nrarp probe | Shifley et el. 2008 | |
| Hes7 cDNA probe | Shifley et el. 2008 | |
| Hes7 intron probe | Shifley et el. 2008 | |
| Hes5 probe | Akazawa et al. 1992 | |
| Software and Algorithms | ||
| GraphPad Prism | GraphPad | |
| Other | ||