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. 2022 Nov 22;164(1):bqac191. doi: 10.1210/endocr/bqac191

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Figure 5. — Arachidonic acid (AA) induces Nrf2 transcription of AKR1C2. (A) Time course experiment of transcriptional upregulation of AKR1C2 (left panel) and AKR1C3 (right panel) after treatment of C4-2 cells with 50 μM AA for the indicated times. (B, C) Top panels: Western blot analysis of Nrf2 in whole-cell lysate (top panel) of C4-2 and LNCaP cells treated with AA. Bottom panels: Nrf2 protein translocation in nuclear and cytoplasmic fractions in C4-2 and LNCaP cells treated with AA. (D) Protein (left panel) and mRNA levels (right panel) of Nrf2, AKR1C2, and AKR1C3 in C4-2 cells stably expressing shCTRL or 2 different shRNAs targeting NFE2L2 (shNRF2 #1 and shNRF2 #2). ***P < 0.001, ****P < 0.0001, ns not significant.