Key resources table
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse monoclonal anti-MEK1/2 | Cell Signaling Technology | Cat#4694 (L38C12) |
| Rabbit monoclonal anti-Phospho-MEK1/2 (Ser217/221) | Cell Signaling Technology | Cat#9154 (41G9) |
| Abcam | Cat#4762 | |
| Rabbit monoclonal anti-Phospho-SEK1/MKK4 (Ser257) | Cell Signaling Technology | Cat#4514S (C36C11) |
| Mouse monoclonal anti-HSC 70 | Santa Cruz Biotechnology | Cat#7298 |
| IRDye 800CW Donkey anti-Rabbit IgG | Licor | Cat#926-32213 |
| IRDye 680RD Goat anti-Mouse IgG | Licor | Cat#926-68070 |
| Rabbit monoclonal anti-Phospho-MKK3 (Ser189) /MKK6 (Ser207) | Cell Signaling Technology | Cat#9231 |
| Rabbit polyclonal anti-COT | Genetex | Cat#GTX102711 [N3C3] |
| Rabbit monoclonal anti-TAK1 | Cell Signaling Technology | Cat#5206 (D94D7) |
| Chemicals, peptides, and recombinant proteins | ||
| Ulixertinib | Selleck Chemicals | Cat#S7854 |
| Selonsertinib | Selleck Chemicals | Cat#S8292 |
| URMC-099 | Selleck Chemicals | Cat#S7343 |
| TAK-632 | Selleck Chemicals | Cat#S7291 |
| Ponatinib | Selleck Chemicals | Cat#S1490 |
| Gefitinib | Selleck Chemicals | Cat#S1025 |
| BIRB-796 | Selleck Chemicals | Cat#S1574 |
| JNK-XVI (JNK-inh-8) | MedChem Express | Cat#HY-13319 |
| Cot-inhibitor-1 | MedChem Express | Cat#HY-32015 |
| SB-203580 | Sigma-Aldrich | Cat#S8307 |
| 5Z-7-Oxozeaenol | Sigma-Aldrich | Cat#O9890 |
| Batimastat | Selleck Chemicals | Cat#S7155 |
| TNF-α | Gibco | Cat#PHC 3015 |
| IL1β | R&D Systems | Cat#201-LB-005 |
| H2O2 | Thermo Fisher | Cat#H325-500 |
| Sorbitol | Thermo Fisher | Cat#50-70-4 |
| Sodium Chloride | Fisher Scientific | Cat#7647-15-5 |
| KCl | Fisher Scientific | Cat#7447-40-7 |
| Sphingosine-1-phosphate | Fisher Scientific | Cat#13-701 |
| Prostaglandin E2 | Millipore Sigma | Cat#P5640 |
| Oleoyl-L-α-lysophosphatidic acid sodium salt | Millipore Sigma | Cat#L7260 |
| Poly(I:C) | Sigma-Aldrich | Cat#P1530 |
| Cyclohexamide | Sigma-Aldrich | Cat#C4859 |
| EGF | PreproTech | Cat#AF-100-15 |
| Anisomycin | Apexbio | Cat#B6674 |
| EdU | Thermo Fisher | Cat#A10044 |
| Critical commercial assays | ||
| RNeasy Micro Kit | Qiagen | Cat#74004 |
| CellEvent Caspase 3/7 Green Dye | Invitrogen | Cat#C1-423 |
| Deposited data | ||
| Raw and processed RNA sequencing data | This paper | NCBI GEO: GSE213882 |
| Human reference genome NCBI build 38, GRCh38 | Genome Reference Consortium | http://www.ncbi.nlm.nih.gov/projects/genome/assembly/grc/human/ |
| Single cell imaging data | This paper | https://github.com/regotlab/MAP3K-signaling/tree/master DOI: 10.5281/zenodo.7153234 |
| Experimental models: Cell lines | ||
| Human MCF 10A | ATCC | Cat#CRL-10317 |
| Human HEK293FT | Thermo Fisher | Cat#R70007 |
| Oligonucleotides | ||
| See Table S1 | ||
| Recombinant DNA | ||
| pTA30 PGK:ERK-KTR-mCerulean3 (Hygromycin) | Aikin et al., 2020 | N/A |
| pSR1846 PGK:JNK-KTR-mRuby2 (Blasticidin) | Regot et al., 2014 | N/A |
| pAP50 PGK:p38-KTR-mClover | Aikin et al., 2020 | N/A |
| pSR1881 H2B-iRFP | Regot et al., 2014 | N/A |
| pAP53 TRE3G:MEK1DD (puro) | Aikin et al., 2020 | N/A |
| pHC141 TRE3G:MEK2DD (puro) | Aikin et al., 2020 | N/A |
| pYL49 TRE3G:MAP2K7EEEdeltaN (puro) | This paper | N/A |
| pYL47 TRE3G:MAP2K4EEdeltaN (puro) | This paper | N/A |
| pAP54 TRE3G:MAP2K5DD (puro) | This paper | N/A |
| pAP55 TRE3G:MAP2K3DD (puro) | Aikin et al., 2020 | N/A |
| pAP128 TRE3G:MAP3K8 (puro) | This paper | N/A |
| pAP107 TRE3G:ASK1 (puro) | This paper | N/A |
| pAP131 TRE3G:TAK1 (puro) | This paper | N/A |
| pAP124 TRE3G:YSK4 (puro) | This paper | N/A |
| pAP122 TRE3G:MAP3K15 (puro) | This paper | N/A |
| pAP148: TRE3G:TNNI3K (puro) | This paper | N/A |
| pAP116: TRE3G:ILK (puro) | This paper | N/A |
| pAP95: TRE3G:MEKK2 (puro) | This paper | N/A |
| pAP115: TRE3G:ARAF (puro) | This paper | N/A |
| pAP111: TRE3G:MAP3K14 (puro) | This paper | N/A |
| pAP112: TRE3G:TAOK3 (puro) | This paper | N/A |
| pAP114: TRE3G:MAP3K12 (puro) | This paper | N/A |
| pAP109: TRE3G:MAP3K13 (puro) | This paper | N/A |
| pAP113: TRE3G:MAP3K11 (puro) | This paper | N/A |
| pAP93: TRE3G:MAP3K5 kinase domain (puro) | This paper | N/A |
| pAP96:TRE3G:MEKK2 kinase domain (puro) | This paper | N/A |
| DHB-mVenus | Spencer et al., 2013 | Addgene Cat#136461 |
| pTA70 ADAM17 CRISPR KO Assembly 1 (Neomycin) | Aikin et al., 2020 | |
| pAP177 MAP3K8 CRISPR KO Assembly 1 (Neomycin) | This paper | |
| pAP194 MAP3K7 CRISPR KO Assembly 2 (Neomycin) | This paper | |
| Software and algorithms | ||
| MATLAB | MathWorks | www.mathworks.com |
| Metamorph | Molecular Devices | https://www.metamorphsoftware.com/ |
| Python | Python Software Foundation | www.python.org |
| Neural Net Segmentation | This paper | DOI: 10.5281/zenodo.7153234 |
| CellProfiler | Broad Institute | Cellprofiler.org |