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Surgical procedure. Rat skulls sterilization and shaving (A), skin and periosteum slitting (B), skull bone perforating by dental drill (C), Critical sized calvarial defect (8 mm-diameter) (D), Implanting of bare scaffolds, PTFE/PVA and PTFE/PVA/GO, and cell containing scaffolds, PTFE/PVA/Cell and PTFE/PVA/GO/Cell (E, F), skin incision suturing (G), fixed sample (H).
