Fig. 7.

Decreased geranylgeranylation of RhoA and increased expression of p21 in Ggps1 cKO mice. A Western blotting for Ggpps and non-prenylated Rap1a. There was a significant reduction on Ggpps levels in Ggps1 cKO mice (**P = 0.010; n = 3 mice per group). There was a significant increase on protein levels of non-prenylated Rap1a in Ggps1 cKO mice compared with controls at P0 (**P = 5.3 × 10^–4; n = 3 mice per group). β-actin was used as the internal control. Raw data were shown in Additional file 4: Fig. S4A. B Quantitative RT-PCR analysis on p21. There was a significant increase on p21 mRNA levels in Ggps1 cKO mice compared with controls at P0 (***P = 1.5 × 10^–4; n = 5 mice per group per age). C Western blotting results for p21. There was a significant increase in Ggps1 cKO mice at P0 (**P = 0.001; n = 3 mice per group). Raw data were shown in Additional file 4: Fig. S4B. D Western blotting for Rho A. Protein lysates for total (T) cerebellum, membrane (M) and cytosol (C) fractionations were prepared from mice at P0. Raw data were shown in Additional file 4: Fig. S4C. E Relative levels of RhoA in different lysates at P0. There was no significant difference on levels of total RhoA between control and Ggps1 cKO mice (P > 0.05, n = 5 mice per group). There were significant differences on RhoA levels in the cytosol and membrane fractionations between control and Ggps1 cKO mice (*P = 0.010, *P = 0.039, respectively; n = 5 mice per group). β–actin and ATP1A1 were used as the internal control for the cytosol and membrane fractionations, respectively