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Dose–responses observed for nitrile- or alkyne-bearing SARS-CoV-2 Mpro inhibitors. Representative dose–response curves of Mpro inhibitors shown in (a) Table 1, (b) Table 2, and (c) Table 3. High Z′-factors66 (>0.5 for each inhibition plate) indicate excellent solid-phase extraction coupled to mass spectrometry (SPE-MS) assay quality (Supporting Figure S5).
