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Western Blot Analysis of EMT Marker Proteins and VEGFA. CCA cells were treated with indicated concentrations of BBR for 24 h before subjecting to SDS-PAGE and Western blotting. (a) Western blot, (b) Quantitative analysis of band intensities by giving the value of the untreated control as 1. Expression of GAPDH protein was used as a loading control. Data are mean ± SD from three independent experiments. *P < 0.05, **P < 0.01
