Figure 4.

Effect of BBR Treatment on the EGF/EGFR Activation. (a) Molecular docking simulates the binding of BBR to the ATP-binding pocket of EGFR, Erk1 and Akt, and to the SH2 domain of STAT3, suggesting the inhibition of BBR on the functions of these molecules. (b) The Western blotting demonstrates the effect of BBR on EGFR, its downstream effectors and the target proteins. (c) The quantitative analyses of the phosphorylated form and total form of EGFR, VEGFA and Slug; and (d) the phosphorylated form and total form of pAkt and Akt. The data are presented by giving those of the EGF treated cells as 1. *P < 0.05, **P < 0.01