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. 2022 Aug 17;42(6):980–987. doi: 10.19852/j.cnki.jtcm.20220817.001

Figure 4. Immunohistochemical assessment the expression of NF-κBp65 in control, RA, and moxibustion group.

Figure 4

A-C: Immunohistochemistry (S-P) analysis (× 400). A to C representative images show the expression of NF-κBp65 in synovial tissues of control (A), RA (B) and moxibustion (C) group. Black arrow indicates the position expression of NF-κBp65. D represented the statistical analysis of the expression of NF-κBp65. n =10 for each group. RA group and Moxa group were established by injecting FCA (0.5 mL/kg) into the bilateral posterior knee joints. Control group was injected with the same volume sterile normal saline in the same way. Moxa group was treated by moxa-burning heat on Zusanli (ST36) and Shenshu (BL23) once 1day for 3 courses after FCA injection. Other two groups were fixed with the same method for the same time without moxibustion. Data were expressed as the mean ± standard deviation. Compared with the Control group, aP < 0.05; compared with the RA group, bP < 0.05. NF-κB: nuclear factor-κ-gene binding; RA: rheumatoid arthritis; FCA: Freund’s complete adjuvant.