Table 2.
Genotoxicity activity of crude MESD stem bark, leaves and its fractions
| Test Sample | Conc
(mg/L) |
Stem bark | Leaves | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Level of Damage | DI | Level of Damage | DI | ||||||||||
| 0 | 1 | 2 | 3 | 0 | 1 | 2 | 3 | ||||||
| MESD | 20 | 68.46±0.62 | 21.59±0.89 | 7.23±1.83 | 2.72±0.81 | 44.21 | 64.68±1.23 | 29.27±0.55 | 5.30±0.38 | 1.75±0.19 | 45.12 | ||
| 100 | 62.00±0.50 | 19.91±1.80 | 11.17±0.37 | 6.92±0.11 | 57.01 | 61.70±0.42 | 25.61±1.80 | 9.87±0.97 | 2.82±1.81 | 53.81 | |||
| 200 | 60.29±1.73 | 30.55±0.22 | 6.90±0.01 | 2.26±1.48 | 45.13 | 59.92±0.27 | 31.65±0.79 | 7.11±0.61 | 1.32±0.22 | 49.83 | |||
| n-hexane | 20 | 77.13±0.97 | 21.59±1.29 | 1.30±0.12 | 0.98±1.18 | 27.13 | 79.22±0.54 | 18.71±0.58 | 1.10±0.10 | 0.97±0.18 | 23.82 | ||
| 100 | 72.40±1.30 | 20.13±0.92 | 5.15±0.74 | 1.73±0.45 | 35.62 | 75.39±0.61 | 16.87±0.67 | 6.12±0.26 | 0.62±0.20 | 30.97 | |||
| 200 | 74.25±1.64 | 18.61±0.88 | 6.14±0.96 | 1.00±0.92 | 33.89 | 71.64±1.98 | 22.43±0.82 | 4.88±0.42 | 1.05±0.23 | 35.34 | |||
| DCM | 20 | 60.61±0.79 | 33.67±0.78 | 4.60±0.54 | 1.12±1.14 | 46.23 | 50.78±1.77 | 40.88±1.09 | 7.16±0.94 | 1.18±0.12 | 58.74 | ||
| 100 | 54.30±0.05 | 38.12±1.11 | 5.75±1.19 | 1.59±0.86 | 54.39 | 44.20±0.75 | 37.82±1.39 | 15.22±1.00 | 2.76±1.01 | 76.54 | |||
| 200 | 52.23±0.33 | 25.45±0.22 | 16.77±0.12 | 5.55±1.76 | 75.64 | 45.29±0.34 | 38.12±0.95 | 14.12±0.93 | 2.78±0.13 | 74.70 | |||
| EtOAc | 20 | 59.32±0.22 | 29.56±1.79 | 09.31±0.34 | 1.87±0.54 | 53.79 | 58.90±1.29 | 30.07±0.57 | 8.12±0.66 | 2.91±0.46 | 55.04 | ||
| 100 | 52.10±0.50 | 40.22±0.89 | 5.20±0.87 | 2.48±0.67 | 58.06 | 50.90±0.88 | 43.76±0.23 | 4.26±0.22 | 1.08±0.33 | 55.52 | |||
| 200 | 53.65±0.88 | 32.72±0.42 | 11.87±0.12 | 1.24±1.65 | 60.18 | 47.05±0.12 | 42.12±0.40 | 9.00±1.00 | 1.83±0.64 | 65.61 | |||
| n-butanol | 20 | 86.36±0.34 | 10.22±0.80 | 1.96±1.77 | 1.50±0.28 | 18.64 | 83.02±0.68 | 13.72±0.58 | 2.05±0.43 | 1.21±0.20 | 21.45 | ||
| 100 | 79.19±1.31 | 16.11±1.83 | 2.99±0.09 | 1.71±0.78 | 27.22 | 80.99±1.69 | 14.51±0.84 | 3.29±0.28 | 1.21±0.16 | 24.72 | |||
| 200 | 77.33±0.66 | 14.12±0.73 | 6.92±0.01 | 1.63±0.86 | 32.85 | 77.98±1.93 | 17.12±0.56 | 3.22±0.67 | 1.68±0.77 | 28.60 | |||
| Aqueous | 20 | 88.66±1.55 | 7.32±0.23 | 3.02±0.51 | 1.00±0.58 | 16.36 | 84.01±1.89 | 10.67±1.80 | 4.06±0.22 | 1.10±0.47 | 22.09 | ||
| 100 | 78.29±0.67 | 15.31±0.99 | 5.34±1.37 | 1.06±0.22 | 29.17 | 77.41±1.34 | 18.58±1.36 | 3.12±0.47 | 0.81±0.12 | 27.25 | |||
| 200 | 79.54±0.52 | 11.79±1.44 | 5.88±0.01 | 3.66±0.14 | 34.53 | 72.32±1.90 | 21.32±1.06 | 4.19±0.60 | 2.17±1.00 | 36.21 | |||
| -ive control | 0 | 84.05±1.20 | 13.67±0.43 | 2.28±0.63 | 0.00±0.00 | 18.23 | 84.05±1.20 | 13.67±0.43 | 2.28±0.63 | 0.00±0.00 | 18.23 | ||
| +ive control | 4mM | 11.00±2.03 | 48.21±0.99 | 25.22±1.32 | 15.59±2.63 | 145.42 | 11.0±2.03 | 48.21±0.99 | 25.22±1.32 | 15.59±2.63 | 145.42 | ||
Notes: MESD: methanolic extract of Sterculia diversifolia; DCM: dichloromethane; DI: damage index; EtOAc: ethyl acetate.